| Products/Services Used | Details | Operation |
|---|---|---|
| Proteins, Expression, Isolation and Analysis | … After incubation on ice for 30 min, samples were subjected to ultrasonic treatment and centrifuged at 10,000 × g for 30 min at 4°C. GST-PLDδ was then affinity purified using glutathione resin (GenScript, Nanjing, China) and subjected to SDS-PAGE … | Get A Quote |
The actin cytoskeleton plays pivotal roles in pollen tube growth by regulating organelle movement, cytoplasmic streaming, and vesicle trafficking. Previous studies have reported that plasma membrane-localized phospholipase Dδ (PLDδ) binds to cortical microtubules and negatively regulates plant stress tolerance. However, it remains unknown whether or how PLDδ regulates microfilament organization. In this study, we found that loss of function led to a significant increase in pollen tube growth, whereas overexpression resulted in pollen tube growth inhibition. We also found that wild-type , rather than Arg 622-mutated , complemented the phenotype in pollen tubes. biochemical assays demonstrated that PLDδ b... More
